1MP5
Y177F VARIANT OF S. ENTERICA RmlA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Collection date | 2001-06-08 |
Wavelength(s) | 1.1395 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.700, 111.220, 131.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.750 |
Rwork | 0.230 |
R-free | 0.29200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iin |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.340 * |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.750 * |
Rmerge | 0.061 * |
Number of reflections | 43514 |
Completeness [%] | 98.3 * |
Redundancy | 5. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | pH 7.0, temperature 100K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | HEPES | 5 (mM) | pH7.4 |
4 | 1 | reservoir | ammonium sulfate | 1.8-1.9 (M) | |
5 | 1 | reservoir | isopropanol | 5-7.5 (%(v/v)) |