1MP4
W224H VARIANT OF S. ENTERICA RmlA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Collection date | 2001-06-08 |
Wavelength(s) | 1.1395 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 88.958, 88.958, 329.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 * - 2.200 |
R-factor | 0.2838811 |
Rwork | 0.257 |
R-free | 0.28900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iin |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.260 * |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 * |
Rmerge | 0.061 * |
Number of reflections | 43514 |
Completeness [%] | 94.3 * |
Redundancy | 17.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | pH 7.0, temperature 100K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | HEPES | 5 (mM) | pH7.4 |
4 | 1 | reservoir | sodium phosphate | 0.8 (M) | |
5 | 1 | reservoir | potassium phosphate | 0.8 (M) | |
6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |