1MP3

L89T VARIANT OF S. ENTERICA RmlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Collection date	2001-03-29
Wavelength(s)	1.0720
Spacegroup name	P 43 21 2
Unit cell lengths	120.015, 120.015, 93.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.210 *
Rwork	0.240
R-free	0.28100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB EMTRY 1IIN
RMSD bond length	0.008 *
RMSD bond angle	1.300 *

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.049 *
Number of reflections	33576
Completeness [%]	95.0 *
Redundancy	4. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		pH 7.0, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	HEPES	5 (mM)	pH7.4
4	1	reservoir	ammonium phosphate	2.0 (M)
5	1	reservoir		10 (mM)
6	1	reservoir	Tris	0.1 (M)	pH8.5