1MOZ

ADP-ribosylation factor-like 1 (ARL1) from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	104.250, 104.250, 45.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 - 3.170
R-factor	0.24
Rwork	0.206
R-free	0.24200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrf
RMSD bond length	0.008
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	3.220
High resolution limit [Å]	3.170	3.170
Rmerge	0.071	0.217
Total number of observations	30535 *
Number of reflections	9179
<I/σ(I)>	16
Completeness [%]	97.5	94.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	polyethylene glycol 8000, TRIS, GDP, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	glycerol	15 (%)
3	1	reservoir	Tris	0.1 (M)	pH8.5
4	1	drop	protein	1 (mM)