1MOV
Crystal structure of Coral protein mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 131.391, 131.391, 150.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mou |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.335 * |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.335 |
| Total number of observations | 176177 * | |
| Number of reflections | 25988 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 * | 293 | peg, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 10-15 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.2 |
| 4 | 1 | reservoir | potassium iodide | 0.2 (M) |
