1MOR

ISOMERASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH GLUCOSE 6-PHOSPHATE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-08-28
Detector	MAR scanner 300 mm plate
Spacegroup name	H 3 2
Unit cell lengths	146.100, 146.100, 173.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.191 *
Rwork	0.191
R-free	0.22400 *
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BLANC
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.084	0.593
Number of reflections	55613 *
<I/σ(I)>	14.3	1.8
Completeness [%]	99.2	98.8
Redundancy	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	30 (%)
2	1	1	MES	0.1 (M)
3	1	1	ammonium sulfate	0.5 (M)
4	1	1	Fru-6P	10 (mM)