1MOJ
Crystal structure of an archaeal dps-homologue from Halobacterium salinarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9762 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 90.747, 90.747, 149.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.880 - 1.900 |
R-factor | 0.147 |
Rwork | 0.147 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dps |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.990 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.096 | 0.450 |
Number of reflections | 63709 | |
<I/σ(I)> | 12.3 | 4.1 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG 400, Tris/HCl, magnesium chloride, sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |