1MOJ
Crystal structure of an archaeal dps-homologue from Halobacterium salinarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 90.747, 90.747, 149.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.880 - 1.900 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dps |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.450 |
| Number of reflections | 63709 | |
| <I/σ(I)> | 12.3 | 4.1 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG 400, Tris/HCl, magnesium chloride, sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
