1MO1
CRYSTAL STRUCTURE AT 1.8 ANGSTROMS OF SELENO METHIONYLED CRH, THE BACILLUS SUBTILIS CATABOLITE REPRESSION CONTAINING PROTEIN CRH REVEALS AN UNEXPECTED SWAPPING DOMAIN AS AN UNTERTWINNED DIMER
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-03-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9763,0.9798,0.9801 |
Spacegroup name | P 43 |
Unit cell lengths | 68.240, 68.240, 115.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.750 - 1.800 |
Rwork | 0.164 |
R-free | 0.19400 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.750 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.150 |
Number of reflections | 46777 | |
<I/σ(I)> | 15.6 | 3.6 |
Completeness [%] | 96.0 | 94 |
Redundancy | 4.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | ammonium sulfate, PEG 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |