1MNZ
Atomic structure of Glucose isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.880, 99.680, 102.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 0.990 |
| R-factor | 0.123 |
| Rwork | 0.123 |
| R-free | 0.13400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xib |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 251156 | |
| <I/σ(I)> | 26.1 | 4.1 |
| Completeness [%] | 98.1 | 96.5 |
| Redundancy | 28.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 295 | MPD, Magnesium chloride, Tris base, pH 7.5, EVAPORATION, temperature 295K |
