1MNV
Actinomycin D binding to ATGCTGCAT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 110 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 47.240, 47.240, 69.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.29500 |
Structure solution method | MAD |
RMSD bond length | 0.018 * |
RMSD bond angle | 3.100 * |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.720 |
High resolution limit [Å] | 2.600 | 2.500 * |
Rmerge | 0.055 | 0.139 |
Number of reflections | 2650 * | |
<I/σ(I)> | 29.1 | 14.4 |
Completeness [%] | 96.9 | 99 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 4 * | SODIUM-CACODYLATE BUFFER (PH 6.0), 10 MM BACL2, 3 MM SPERMINE, 4 % MPD SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 323K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium-cacodylate buffer | ||
2 | 1 | 1 | BaCl2 | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | BaCl2 | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA single strand | 1.3 (mM) | |
2 | 1 | drop | ActD | 1.3 (mM) | |
3 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
4 | 1 | drop | 10 (mM) | ||
5 | 1 | drop | spermine | 3 (mM) | |
6 | 1 | drop | 4 (%) | ||
7 | 1 | reservoir | MPD | 50 (%) |