1MNG

STRUCTURE-FUNCTION IN E. COLI IRON SUPEROXIDE DISMUTASE: COMPARISONS WITH THE MANGANESE ENZYME FROM T. THERMOPHILUS

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	146.800, 146.800, 55.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.179
Rwork	0.179
RMSD bond length	0.022
RMSD bond angle	1.984
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.077 *
Number of reflections	52673 *
Redundancy	6.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	drop	phosphate	5 (mM)
3	1	drop	ammonium sulfate
4	1	reservoir	ammonium sulfate	40 (%sat)
5	1	reservoir	potassium acid phosphate	0.5 (M)