1MN8
Structure of Moloney Murine Leukaemia Virus Matrix Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.800, 49.500, 50.800 |
| Unit cell angles | 71.90, 81.90, 80.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-free | 0.16900 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.062 | |
| Number of reflections | 141760 | |
| <I/σ(I)> | 18.1 | 0.9 |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na pH 7.5, 1.4M sodium citrate, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na pH 7.5, 1.4M sodium citrate, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-Na | 0.1 (M) | pH7.5 |
| 3 | 1 | reservoir | sodium citrate | 1.4 (M) | |
| 4 | 1 | reservoir | 100 (mM) |
