1MN4
Structure of Ndt80 (Residues 59-340) DNA-binding domain core
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2002-05-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9563 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.880, 41.930, 164.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.200 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.280 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.076 * | 0.221 * |
Total number of observations | 144249 * | |
Number of reflections | 12292 * | |
<I/σ(I)> | 20.9 | 7.9 |
Completeness [%] | 98.1 | 100 |
Redundancy | 11.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 20 * | PEG 4000, sodium acetate, magnesium chloride, spermine, 20mer DNA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 600 (mM) | |
3 | 1 | reservoir | 2 (mM) | ||
4 | 1 | reservoir | spermine | 1 (mM) | |
5 | 1 | reservoir | PEG4000 | 25 (%) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.5 |