1MN3
Cue domain of yeast Vps9p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-07-13 |
| Detector | SBC-2 |
| Wavelength(s) | 0.95645,0.97910,0.97924 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.487, 70.487, 61.384 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.400 * - 2.300 |
| Rwork | 0.244 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 * | 0.408 * |
| Number of reflections | 4327 * | 415 * |
| <I/σ(I)> | 39.5 | 6.8 |
| Completeness [%] | 99.8 | 99.8 * |
| Redundancy | 23.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 293 | Ammonium Sulfate, Tris buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris | 20 (mM) | pH7.7 |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.9-2.1 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2-8.8 |
