1MMV
Rat neuronal NOS heme domain with NG-propyl-L-arginine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.080 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.590, 110.360, 164.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.910 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.25600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.693 | |
| Number of reflections | 62537 | |
| <I/σ(I)> | 8.6 | 1.7 |
| Completeness [%] | 96.8 | |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 280 | PEG 4000, MES, ammonium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP at 280K |
