1MMS
Crystal structure of the ribosomal PROTEIN L11-RNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Wavelength(s) | 0.980,1.010 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.890, 84.260, 155.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.570 |
| R-factor | 0.228 * |
| Rwork | 0.219 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.730 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 49313 | |
| <I/σ(I)> | 20 | 4.3 |
| Completeness [%] | 95.5 | 86.2 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | 25% GLYCEROL, 15% PEG 4000, 50 MM TRIS PH 7.5, 50 MM MGCL2, 20 MM CDCL2, 0.2 M KCL, 1 MM DITHIOTHREITOL, 4 DEGREES C, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | GLYCEROL | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | TRIS | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | CDCL2 | ||
| 6 | 1 | 1 | KCL | ||
| 7 | 1 | 1 | DITHIOTHREITOL | ||
| 8 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.15 (mM) | |
| 2 | 1 | reservoir | glycerol | 25 (%) | |
| 3 | 1 | reservoir | PEG4000 | 15 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | Tris | 50 (mM) |
