1MMS
Crystal structure of the ribosomal PROTEIN L11-RNA complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | BRANDEIS |
Wavelength(s) | 0.980,1.010 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.890, 84.260, 155.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.570 |
R-factor | 0.228 * |
Rwork | 0.219 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.730 |
High resolution limit [Å] | 2.570 | 2.570 |
Number of reflections | 49313 | |
<I/σ(I)> | 20 | 4.3 |
Completeness [%] | 95.5 | 86.2 |
Redundancy | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | 25% GLYCEROL, 15% PEG 4000, 50 MM TRIS PH 7.5, 50 MM MGCL2, 20 MM CDCL2, 0.2 M KCL, 1 MM DITHIOTHREITOL, 4 DEGREES C, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | GLYCEROL | ||
2 | 1 | 1 | PEG 4000 | ||
3 | 1 | 1 | TRIS | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | CDCL2 | ||
6 | 1 | 1 | KCL | ||
7 | 1 | 1 | DITHIOTHREITOL | ||
8 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.15 (mM) | |
2 | 1 | reservoir | glycerol | 25 (%) | |
3 | 1 | reservoir | PEG4000 | 15 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | 50 (mM) | ||
6 | 1 | reservoir | 20 (mM) | ||
7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
8 | 1 | reservoir | Tris | 50 (mM) |