1MMI
E. COLI DNA POLYMERASE BETA SUBUNIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.012, 66.595, 80.779 |
| Unit cell angles | 90.00, 114.02, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 * |
| R-factor | 0.19119 |
| Rwork | 0.188 |
| R-free | 0.24460 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pol |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.056 | 0.467 |
| Number of reflections | 62043 | 6056 * |
| <I/σ(I)> | 1.75 | |
| Completeness [%] | 93.4 * | 91.7 |
| Redundancy | 1.92 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 294 * | 100 mM Na/H MES, 50 to 60 mM CaCl2, 30% v/v PEG 400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 45-60 (mg/ml) | |
| 2 | 1 | reservoir | sodium MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | 50-60 (mM) | ||
| 4 | 1 | reservoir | PEG400 | 30 (%(v/v)) |
