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1MMF

Crystal structure of substrate free form of glycerol dehydratase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 5ID-B
Synchrotron siteAPS
Beamline5ID-B
Temperature [K]100
Detector technologyCCD
Collection date1999-08-11
DetectorMARRESEARCH
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths93.700, 110.070, 114.690
Unit cell angles90.00, 107.64, 90.00
Refinement procedure
Resolution30.000 - 2.500
Rwork0.228
R-free0.26900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1dio
RMSD bond length0.008
RMSD bond angle1.370
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.540
High resolution limit [Å]2.5002.500
Rmerge0.0720.140
Total number of observations302300

*

Number of reflections67426
<I/σ(I)>11.63.1
Completeness [%]87.750.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

*

298Amonium sulfate, RbCl2, CdCl2, cyanocobalamin, CHES, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirammonium sulfate1.1-1.3 (M)
21reservoir0.2 (M)
31reservoir0.2 (mM)
41reservoircyanocobalamin0.2 (M)
51reservoirCHES-KOH0.1 (M)pH9.4-9.7
61dropprotein8 (mg/ml)
71dropHEPES-KOH50 (mM)pH7.5

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PDB entries from 2025-01-29

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