1MME
THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 1994-11-03 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.980, 64.980, 138.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.100 |
R-factor | 0.251 |
Rwork | 0.251 |
R-free | 0.29300 |
RMSD bond length | 0.010 |
RMSD bond angle | 16.500 * |
Data reduction software | IPMOSFLM |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 3.100 |
Rmerge | 0.085 |
Number of reflections | 6529 |
Completeness [%] | 99.9 |
Redundancy | 3.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 20 * | pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | PEG 6000 | ||
3 | 1 | 1 | GLYCEROL | ||
4 | 1 | 1 | NH4 CACODYLATE | ||
5 | 1 | 1 | NH4 ACETATE | ||
6 | 1 | 1 | MG ACETATE | ||
7 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ribozyme complex | 0.5 (mM) | |
2 | 1 | drop | ammonium cacodylate | 10 (mM) | |
3 | 1 | reservoir | ammonium cacodylate | 50 (mM) | |
4 | 1 | reservoir | 100 (mM) | ||
5 | 1 | reservoir | 10 (mM) | ||
6 | 1 | reservoir | spermine | 1 (mM) | |
7 | 1 | reservoir | PEG6000 | 23 (%) | |
8 | 1 | reservoir | glycerol | 5 (%) |