1ML0

VIRAL CHEMOKINE BINDING PROTEIN M3 FROM MURINE GAMMAHERPESVIRUS68 IN COMPLEX WITH THE P8A VARIANT OF CC-CHEMOKINE MCP-1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	110
Detector technology	CCD
Collection date	2000-06-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000, 0.9796, 0.9789, 0.9599
Spacegroup name	P 3 2 1
Unit cell lengths	99.200, 99.200, 78.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.370 - 2.800
Rwork	0.201
R-free	0.28200
Structure solution method	MAD AND MOLECULAR REPLACEMENT
Starting model (for MR)	1mkf
RMSD bond length	0.008
RMSD bond angle	25.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.970
High resolution limit [Å]	2.800	3.000 *
Rmerge	0.080 *	0.286 *
Total number of observations	41932 *
Number of reflections	16718 *
<I/σ(I)>	14.5	4.8
Completeness [%]	96.3 *	94.4 *
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.1	293	14% PEG4000 200MM, SODIUM ACETATE, 100MM MAGNESIUM CHLORIDE, pH 4.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	14 (%)
3	1	reservoir	sodium acetate	200 (mM)	pH4.1
4	1	reservoir		100 (mM)