1ML0
VIRAL CHEMOKINE BINDING PROTEIN M3 FROM MURINE GAMMAHERPESVIRUS68 IN COMPLEX WITH THE P8A VARIANT OF CC-CHEMOKINE MCP-1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2000-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000, 0.9796, 0.9789, 0.9599 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 99.200, 99.200, 78.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.370 - 2.800 |
Rwork | 0.201 |
R-free | 0.28200 |
Structure solution method | MAD AND MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mkf |
RMSD bond length | 0.008 |
RMSD bond angle | 25.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.970 |
High resolution limit [Å] | 2.800 | 3.000 * |
Rmerge | 0.080 * | 0.286 * |
Total number of observations | 41932 * | |
Number of reflections | 16718 * | |
<I/σ(I)> | 14.5 | 4.8 |
Completeness [%] | 96.3 * | 94.4 * |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | 14% PEG4000 200MM, SODIUM ACETATE, 100MM MAGNESIUM CHLORIDE, pH 4.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 14 (%) | |
3 | 1 | reservoir | sodium acetate | 200 (mM) | pH4.1 |
4 | 1 | reservoir | 100 (mM) |