1MKY
Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.137, 77.994, 96.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22700 * |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 22.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.074 * | 0.344 * |
Total number of observations | 465562 * | |
Number of reflections | 44093 * | |
<I/σ(I)> | 26.5 | 6.7 |
Completeness [%] | 99.6 | 99.2 * |
Redundancy | 10.6 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 298 | sodium phosphate, potassium phosphate, 3-(cyclohexylamino)-1-propane sulphonic acid (CAPS), pH 10.0, VAPOR DIFFUSION, HANGING DROP at 298K, temperature 298K, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.3 (M) | ||
2 | 1 | reservoir | 0.7 (M) | ||
3 | 1 | reservoir | CAPS | 50 (mM) | pH10.0 |
4 | 1 | reservoir | beta-mercaptoethanol | 15 (mM) | |
5 | 1 | drop | protein | 26 (mg/ml) | |
6 | 1 | drop | 0.8 (M) | ||
7 | 1 | drop | 0.5 (M) | ||
8 | 1 | drop | CAPS | 50 (mM) | pH10.0 |
9 | 1 | drop | beta-mercaptoethanol | 15 (mM) |