1MKR
Crystal Structure of a Mutant Variant of Cytochrome c Peroxidase (Plate like crystals)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 124 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.480, 50.957, 118.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.580 |
| Rwork | 0.194 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | H52Y6 Dataset - PDB Entry - 1MKQ |
| RMSD bond length | 0.005 |
| RMSD bond angle | 2.245 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.049 | 0.394 |
| Total number of observations | 627338 * | |
| Number of reflections | 42967 | |
| <I/σ(I)> | 37.19 | 2.72 |
| Completeness [%] | 94.8 | 87.9 |
| Redundancy | 14.602 | 4.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 4 * | 2-Methyl-2,4-Pentanediol (MPD), Tris-phosphate buffer, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | KPB | 50 (mM) | or Tris-phosphate, pH6.0 |
| 3 | 1 | drop | MPD | 18 (%) | |
| 4 | 1 | reservoir | MPD | 30 (%) |
