Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97946, 0.97955, 0.93927 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.229, 61.076, 95.911 |
| Unit cell angles | 90.00, 110.51, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.200 |
| R-factor | 0.257 |
| Rwork | 0.232 |
| R-free | 0.30000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.299 |
| Number of reflections | 27598 | |
| <I/σ(I)> | 15.9 | 2.5 |
| Completeness [%] | 85.9 | 59.6 |
| Redundancy | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 8000, Glycerol, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.1 (M) | pH7. |
| 2 | 1 | reservoir | PEG8000 | 5 (%) | |
| 3 | 1 | reservoir | glycerol | 5 (%) | |
| 4 | 1 | drop | protein | 9 (mg/ml) |
