1MKI
Crystal Structure of Bacillus Subtilis Probable Glutaminase, APC1040
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-13 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97910, 0.97921, 0.954 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.332, 181.482, 51.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.700 * - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.000 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.502 |
| Number of reflections | 46201 | |
| <I/σ(I)> | 5.4 | 3.7 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 10.2 | 9.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 21 * | PEG 1500, HEPES, sodium chloride, Glycerol, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3350 | 25 (%) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | drop | sodium HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | PEG1500 | 30 (%) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | glycerol | 5 (%) | |
| 7 | 1 | reservoir | dithiothreitol | 0.1 (mM) | |
| 8 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
