1MKH

C-terminal domain of methionyl-tRNA synthetase from Pyrococcus abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-07
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 32 2 1
Unit cell lengths	38.328, 38.328, 161.606
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.010
R-factor	0.28
Rwork	0.275
R-free	0.28900 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.530 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.600	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.052 *	0.208 *
Number of reflections	9286 *
Completeness [%]	97.3	75.9
Redundancy	3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7	297	peg 8000, potassium phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP at 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	20 (%)
2	1	1	potassium phosphate	50 (mM)