1MK5

Wildtype Core-Streptavidin with Biotin at 1.4A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	I 2 2 2
Unit cell lengths	46.305, 93.414, 103.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.1279
R-free	0.18900
Structure solution method	ISOMORPHOUS
Starting model (for MR)	1df8
RMSD bond length	0.012
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.043	0.200
Total number of observations	383001 *
Number of reflections	44171
<I/σ(I)>	30.5	4.5
Completeness [%]	98.8	93.4
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	ammonium sulphate, sodium acetate, sodium chloride, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	reservoir	ammonium sulfate	35 (%)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.5
4	1	reservoir		0.2 (M)