1MK4
Structure of Protein of Unknown Function YqjY from Bacillus subtilis, Probable Acetyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-31 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.613, 103.613, 72.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.090 - 1.700 |
| Rwork | 0.220 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.090 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.460 |
| Number of reflections | 48073 | |
| <I/σ(I)> | 27 | 4.59 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 7.14 | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
