1MJW

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D42N

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-06
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	110.300, 110.300, 154.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.950
R-factor	0.191 *
Rwork	0.191
R-free	0.26600
RMSD bond length	0.016
RMSD bond angle	0.039
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.950 *
Rmerge	0.057 *
Total number of observations	2581560 *
Number of reflections	259100 *
Completeness [%]	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Avaeva, S., (1997) FEBS Lett., 410, 502. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.1 (M)
2	1	drop	ammonium sulfate	35 (%)
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	ammonium sulfate	45 (%)