1MJS

MH2 domain of transcriptional factor SMAD3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2000-06-17
Detector	CUSTOM-MADE
Wavelength(s)	1.1
Spacegroup name	P 32
Unit cell lengths	54.325, 54.325, 59.428
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.120 - 1.900 *
Rwork	0.196
R-free	0.20400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SMAD4 active fragment
RMSD bond length	0.010 *
RMSD bond angle	24.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.120	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.061	0.210
Total number of observations	64905 *
Number of reflections	14785 *
<I/σ(I)>	20.2	3.53
Completeness [%]	96.4	77.7 *
Redundancy	4.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium dihydrogen phosphate	700 (mM)
2	1	reservoir	ethylene glycol	20 (%)	pH4.8
3	1	drop	Tris	20 (mM)	pH7.4
4	1	drop		10 (mM)
5	1	drop	EDTA	0.1 (mM)
6	1	drop	dithiothreitol	1 (mM)