1MJM
METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO HALF OF THE CONSENSUS OPERATOR SEQUENCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.960, 64.000, 114.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.400 - 2.200 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cma |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.075 |
| Number of reflections | 60504 | |
| <I/σ(I)> | 8 | 2.8 |
| Completeness [%] | 89.8 | 56.3 |
| Redundancy | 4.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | PROTEIN (10MG/ML) + DNA (3.8MG/ML) WAS CRYSTALLISED FROM 8-14% P4000, 100MM CITRATE BUFFER, PH 5.0-6.0, 20% GLYCEROL, pH 7.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PROTEIN | ||
| 2 | 1 | 1 | DNA | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 1 | CITRATE BUFFER | ||
| 5 | 1 | 1 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 4 (mg/ml) | ||
| 3 | 1 | drop | SAM | 1.5 (mg/ml) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 8 | 1 | reservoir | MPD | 28-38 (%) |
