1MJK

METHIONINE REPRESSOR MUTANT APOREPRESSOR (Q44K) FROM ESCHERICHIA COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-01
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	35.680, 63.570, 44.610
Unit cell angles	90.00, 102.55, 90.00

Refinement procedure

Resolution	23.000 - 2.150
R-factor	0.181
Rwork	0.181
R-free	0.22300
Structure solution method	DIFFERENCE FOURIER METHODS
Starting model (for MR)	1cmb
RMSD bond length	0.007
RMSD bond angle	23.900 *
Data reduction software	XDS
Data scaling software	CCP4
Phasing software	X-PLOR (3.84)
Refinement software	X-PLOR (3.84)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.038	0.038
Number of reflections	10414
<I/σ(I)>	14.9	3.3
Completeness [%]	97.5	87.6
Redundancy	2.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		PROTEIN WAS CRYSTALLIZED FROM 16-28% PEG 600, 1.5% NAIO4, 100MM POTASSIUM PHOSPHATE BUFFER, PH 7.0.