1MJK
METHIONINE REPRESSOR MUTANT APOREPRESSOR (Q44K) FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-01 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.680, 63.570, 44.610 |
| Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.150 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.22300 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1cmb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.900 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.84) |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.038 | 0.038 |
| Number of reflections | 10414 | |
| <I/σ(I)> | 14.9 | 3.3 |
| Completeness [%] | 97.5 | 87.6 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | PROTEIN WAS CRYSTALLIZED FROM 16-28% PEG 600, 1.5% NAIO4, 100MM POTASSIUM PHOSPHATE BUFFER, PH 7.0. |
