1MJI
DETAILED ANALYSIS OF RNA-PROTEIN INTERACTIONS WITHIN THE BACTERIAL RIBOSOMAL PROTEIN L5/5S RRNA COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9680 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.890, 99.600, 65.730 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.500 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | 0.186 |
| Number of reflections | 22852 * | 1908 * |
| <I/σ(I)> | 13.2 | 3.3 |
| Completeness [%] | 99.9 * | 84.1 |
| Redundancy | 3.02 * | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium cacodylate, magnesium acetate, potassium chlorate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | potassium chlorate | ||
| 4 | 1 | 1 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.5 |
| 3 | 1 | drop | magnesium acetate | 100 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | PEG8000 | 10 (%) | |
| 6 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
| 7 | 1 | reservoir | magnesium acetate | 100 (mM) | |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | PEG8000 | 10 (%) |
