1MJF
PUTATIVE SPERMIDINE SYNTHETASE FROM PYROCOCCUS FURIOSUS PFU-132382
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.639, 110.821, 49.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.798 |
| R-factor | 0.20655 |
| Rwork | 0.205 |
| R-free | 0.24156 |
| Structure solution method | AUTOMATED MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1inl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | DENZO (1.9.7) |
| Data scaling software | SCALEPACK (1.9.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 44968 | |
| <I/σ(I)> | 36 | 7 |
| Completeness [%] | 90.9 | 47.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | calcium chloride, PEG400, pH 7.5, microbatch, temperature 291K |
