1MIX

Crystal structure of a FERM domain of Talin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-11
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	55.439, 55.970, 68.242
Unit cell angles	90.00, 111.25, 90.00

Refinement procedure

Resolution	30.000 * - 1.750
R-factor	0.199
Rwork	0.199
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.340	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.053 *	0.140 *
Total number of observations	51869 *
Number of reflections	18077
Completeness [%]	91.5 *	49.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	4 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH7.0
2	1	reservoir	PEG2000 MME	20 (%(w/v))
3	1	drop	protein	10 (mg/ml)
4	1	drop	Tris-HCl	20 (mM)	pH7.0
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	2 (mM)