1MIW
Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1063, 0.9786, 0.9789, 0.9560 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 105.632, 105.632, 184.159 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 * - 3.000 |
| R-factor | 0.2314 |
| Rwork | 0.228 |
| R-free | 0.26300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.755 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.035 | |
| Total number of observations | 241651 * | |
| Number of reflections | 24280 | |
| <I/σ(I)> | 42 | |
| Completeness [%] | 99.8 | 99.9 * |
| Redundancy | 9.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 277 | NaCl, Tris, MgCl2, pH 7.5, MICRODIALYSIS at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8 (mg/ml) | |
| 2 | 1 | 2 | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | 2 | 200 (mM) | ||
| 4 | 1 | 2 | 10 (mM) |
