1MIV
Crystal structure of Bacillus stearothermophilus CCA-adding enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 105.861, 105.861, 183.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.29256 |
| Rwork | 0.289 |
| R-free | 0.32700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.500 | |
| Rmerge | 0.063 | |
| Total number of observations | 124736 * | |
| Number of reflections | 15392 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.4 * | 99.4 * |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 277 | NaCl, Tris, MgCl2, pH 7.5, MICRODIALYSIS at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8 (mg/ml) | |
| 2 | 1 | 2 | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | 2 | 200 (mM) | ||
| 4 | 1 | 2 | 10 (mM) |
