1MIU
Structure of a BRCA2-DSS1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2001-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.950 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 160.509, 228.271, 81.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.26 |
| Rwork | 0.256 |
| R-free | 0.31000 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.046 * | |
| Total number of observations | 136363 * | |
| Number of reflections | 27074 | |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 5.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 4000, Tris-HCl, CaCL2, NaCl, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 6 (%(w/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | 75 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) | pH8.0 |
| 6 | 1 | drop | protein | 20 (mg/ml) | |
| 7 | 1 | drop | Tris-HCl | 20 (mM) | |
| 8 | 1 | drop | 150 (mM) | ||
| 9 | 1 | drop | dithiothreitol | 10 (mM) | pH8.0 |
