1MIH
A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-06-10 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.504, 53.615, 160.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.700 |
R-factor | 0.243 |
Rwork | 0.226 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1FQW |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.230 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.044 * | 0.213 * |
Total number of observations | 87060 * | |
Number of reflections | 13060 | |
<I/σ(I)> | 27.2 | 6.5 |
Completeness [%] | 98.3 * | 98 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 1 (mM) | ||
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 1 (mM) | ||
5 | 1 | reservoir | Tris | 100 (mM) | pH8.4 |
6 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
7 | 1 | reservoir | glycerol | 5 (%(v/v)) |