1MIF
MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-06-14 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.800, 96.800, 106.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 * |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.053 |
| Number of reflections | 18101 |
| Completeness [%] | 82.1 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | drop | PEG400 | 1 (%) | |
| 4 | 1 | drop | HEPES | 0.05 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | PEG400 | 2 (%) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) |
