1MI7
Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-24 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.310, 85.310, 113.968 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.500 |
| R-factor | 0.25505 |
| Rwork | 0.253 |
| R-free | 0.31500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WRP BIOLOGICAL DIMER |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.580 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.052 | 0.169 |
| Total number of observations | 67359 * | |
| Number of reflections | 8304 | 526 * |
| <I/σ(I)> | 24.1 | 2.6 |
| Completeness [%] | 92.5 | 61 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100 mM Na HEPES, 100 mM sodium chloride, 30%(v/v) isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-20 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 30-35 (%(v/v)) | |
| 3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | 100 (mM) |
