1MI1
Crystal Structure of the PH-BEACH Domain of Human Neurobeachin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9764, 0.9793, 0.9791, 0.9686 |
| Spacegroup name | P 61 |
| Unit cell lengths | 179.910, 179.910, 98.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.900 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.079 * | |
| Total number of observations | 355843 * | |
| Number of reflections | 80025 * | |
| Completeness [%] | 99.2 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 4 * | Tris, 10mM DTT, 4% PEG 8000, pH 7.6, VAPOR DIFFUSION, SITTING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 100 (mM) | pH7.6 |
| 2 | 1 | reservoir | PEG8000 | 4 (%(w/v)) | |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | Tris | 20 (mM) | pH8.5 |
| 5 | 1 | drop | 200 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 10 (mM) | |
| 7 | 1 | drop | protein | 30 (mg/ml) |
