1MHQ
Crystal Structure Of Human GGA2 VHS Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.860, 68.110, 74.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.200 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.430 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.440 * |
| Number of reflections | 16016 * | 1214 * |
| <I/σ(I)> | 17.5 | 2.9 |
| Completeness [%] | 95.5 * | 74.7 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG 3350, iso-propanol, beta-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3350 | 10 (%(w/v)) | |
| 2 | 1 | drop | isopropanol | 5 (%) | |
| 3 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | PEG3350 | 20 (%(w/v)) | |
| 5 | 1 | reservoir | isopropanol | 5 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 7 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%(v/v)) |
