1MHN
High resolution crystal structure of the SMN Tudor domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 65 |
| Unit cell lengths | 27.652, 27.652, 110.299 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.947 - 1.800 |
| R-factor | 0.1466 |
| Rwork | 0.144 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.341 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.950 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 4499 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 285 | Sprangers, R., (2003) Acta Crystallogr., D59, 366. * |
