1MHM
Crystal structure of S-adenosylmethionine decarboxylase from potato
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.650, 71.640, 72.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 2.300 |
Rwork | 0.218 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.260 * |
Rmerge | 0.087 * | 0.318 * |
Number of reflections | 17049 * | 1084 * |
Completeness [%] | 90.1 * | 69.6 * |
Redundancy | 3.8 * | 2.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 17 * | trisodium citrate, isopropanol, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | trisodium citrate dihydrate | 0.1 (M) | pH5.6 |
2 | 1 | reservoir | 2-propanol | 20 (%(v/v)) | |
3 | 1 | reservoir | PEG4000 | 20 (%(w/v)) |