1MHK

Crystal Structure Analysis of a 26mer RNA molecule, representing a new RNA motif, the hook-turn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	193
Detector technology	IMAGE PLATE
Collection date	2001-01-31
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	30.169, 62.510, 50.933
Unit cell angles	90.00, 98.76, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.243
Rwork	0.239
R-free	0.27700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mer A-form RNA helix
RMSD bond length	0.006
RMSD bond angle	1.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.128 *	0.342 *
Total number of observations	56979 *
Number of reflections	3175 *
<I/σ(I)>	10.35	2.55
Completeness [%]	98.6	98.5 *
Redundancy	17.69	4.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	19 *	ammonium sulfate, magnesium chloride, spermidine, tris-hydrogen chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP at 292K
1	unknown *	7.5	19 *	ammonium sulfate, magnesium chloride, spermidine, tris-hydrogen chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP at 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	1	MgCl2
3	1	1	spermidine
4	1	1	tris-HCl
5	1	2	(NH4)2SO4
6	1	2	MgCl2
7	1	2	tris-HCl

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		ammonium sulfate	1.8 (M)
4	1		Tris-HCl	50 (mM)	pH7.5