1MH4

maltose binding-a1 homeodomain protein chimera, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.254, 88.705, 96.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.300
Rwork	0.243
R-free	0.29200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb code: 4MBP
RMSD bond length	0.006
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.240 *
Number of reflections	19118
<I/σ(I)>	22.5	3.58
Completeness [%]	96.9 *	83.2
Redundancy	9.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	reservoir	PEG6000	20 (%)