1MH1
SMALL G-PROTEIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 101 |
Collection date | 1994-12 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 46.440, 103.050, 43.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.380 |
R-factor | 0.175 * |
Rwork | 0.175 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 821p |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.543 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.300 | 1.440 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.039 | 0.130 |
Total number of observations | 312780 * | |
Number of reflections | 42134 | |
<I/σ(I)> | 9.8 | 5.6 |
Completeness [%] | 99.2 | 98.8 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3-4 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG8000 | 18-20 (%) | |
6 | 1 | reservoir | Tris-HCl | 25 (mM) | |
7 | 1 | reservoir | 10 (mM) | ||
8 | 1 | reservoir | dithiothreitol | 5 (mM) |