1MGY

Structure of the D85S mutant of bacteriorhodopsin with bromide bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1272
Spacegroup name	C 2 2 21
Unit cell lengths	52.498, 121.326, 73.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.000
Rwork	0.215
R-free	0.23650 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kgb
RMSD bond length	0.007
RMSD bond angle	0.953
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.127 *	0.350 *
Total number of observations	135550 *
Number of reflections	16336
Completeness [%]	94.2	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate, PEG 4000, potassium chloride, mono-olein, pH 4.6, connected bilayer gel, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	2	sodium acetate	100 (mM)	pH4.6
3	1	2		200 (mM)
4	1	2	PEG4000	10 (%)