1MGY
Structure of the D85S mutant of bacteriorhodopsin with bromide bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1272 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.498, 121.326, 73.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| Rwork | 0.215 |
| R-free | 0.23650 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.953 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 * | 0.350 * |
| Total number of observations | 135550 * | |
| Number of reflections | 16336 | |
| Completeness [%] | 94.2 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | 293 | Sodium Acetate, PEG 4000, potassium chloride, mono-olein, pH 4.6, connected bilayer gel, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 2 | sodium acetate | 100 (mM) | pH4.6 |
| 3 | 1 | 2 | 200 (mM) | ||
| 4 | 1 | 2 | PEG4000 | 10 (%) |
