1MGW
Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.100 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.720, 64.720, 69.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.600 * - 2.000 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.21400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rgg |
RMSD bond length | 0.021 |
RMSD bond angle | 1.831 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 2.020 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.306 |
Number of reflections | 11799 | |
<I/σ(I)> | 31.6 | 5.8 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 9.5 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 * | 293 | Tris, HCl, HEPES, lithium sulphate, pH 7.6, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.10 (M) | pH7.6 |
2 | 1 | reservoir | 1.6 (M) | ||
3 | 1 | drop | Tris-HCl | 0.05 (M) | pH8.2 |
4 | 1 | drop | protein | 7.5 (mg/ml) | |
5 | 1 | drop | HEPES | 0.05 (M) | |
6 | 1 | drop | 0.8 (M) |