1MGR
Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.050, 34.050, 147.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 | 0.119 |
| Number of reflections | 10355 | |
| <I/σ(I)> | 47.6 | 14 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | sodium acetate, calcium acetate, acetic acid, MPD, ammonium sulphate, pH 4.2, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 10 (%(v/v)) | |
| 2 | 1 | reservoir | ammonium sulfate | 40 (mM) | |
| 3 | 1 | drop | sodium acetate | 20 (mM) | pH4.2 |
| 4 | 1 | drop | calcium acetate | 2 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | MPD | 5 (%(v/v)) | |
| 7 | 1 | drop | ammonium sulfate | 20 (mM) |
